Interaction between culture filtrates of Fusarium culmorum isolates and some root lesion nematodes

This study was conducted with root lesion nematodes Pratylenchus penetrans, P. thornei and neglectus three isolates of Fusarium culmorum culture filtrates (ISP, B4, FC5) in vitro conditions. The were diluted 1/0,1/10, 1/20, 1/30, 1/40, 1/50, 1/60, 1/70, 1/80, 90, 1/100 1.5 ml microtubes, 250 µl adult+larvae placed each microtubes that containing different concentrations filtrate 50 μl purified water together micropipette incubated at 25±1°C. Nematode mortality determined after 24 h, 48 h 72 h. As a result, effect FC5 to be high on nematode species. B4 ISP caused more deaths penetrans than P.thornei neglectus. It has been found rate increase over time vitro. lowest rates generally 1/90, while the highest 1/0 1/10. No difference detected between F. concentration. In study, antagonistic relationship from